Journal article
φxANES: In vivo imaging of metal-protein coordination environments
SA James, DJ Hare, NL Jenkins, MD De Jonge, AI Bush, G McColl
Scientific Reports | NATURE PORTFOLIO | Published : 2016
DOI: 10.1038/srep20350
Abstract
We have developed an X-ray absorption near edge structure spectroscopy method using fluorescence detection for visualizing in vivo coordination environments of metals in biological specimens. This approach, which we term fluorescence imaging XANES (φXANES), allows us to spatially depict metal-protein associations in a native, hydrated state whilst avoiding intrinsic chemical damage from radiation. This method was validated using iron-challenged Caenorhabditis elegans to observe marked alterations in redox environment.
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Awarded by NIH Office of the Director
Funding Acknowledgements
We thank Daryl L. Howard, David Paterson, and Peter Kappen (Australian Synchrotron) for experimental assistance and discussions. Parts of this research were undertaken on the XFM and XAS beamlines at the Australian Synchrotron. We acknowledge the Caenorhabditis Genetics Center of the US National Institutes of Health - Office of Research Infrastructure Programs (P40OD010440) for materials. The Australian Research Council Discovery Projects scheme (DP130100357), National Health and Medical Research Council and the Victorian Government's Operational Infrastructure Support Program funded this work.